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Treatment scheme for in vivo study. B. Mean tumor volume over the course of treatment in untreated, cisplatin, vitamin D and combination groups. C. Representative immunohistochemical (IHC) staining <t>of</t> <t>ALDH1A1,</t> RAR, RXR, <t>VDR</t> in tumors collected at the end of study, with corresponding quantification shown on the right (n=4), Scale bar, 100µm. D. Expression of stemness associated genes was determined by RT-qPCR analysis in untreated, cisplatin, vitamin D and combination treated tumors. All experiments were performed in biological triplicate unless stated otherwise, +/- SEM, P *<0.5, P **<0.01, P ***<0.0001, P ****<0.00001.
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Treatment scheme for in vivo study. B. Mean tumor volume over the course of treatment in untreated, cisplatin, vitamin D and combination groups. C. Representative immunohistochemical (IHC) staining <t>of</t> <t>ALDH1A1,</t> RAR, RXR, <t>VDR</t> in tumors collected at the end of study, with corresponding quantification shown on the right (n=4), Scale bar, 100µm. D. Expression of stemness associated genes was determined by RT-qPCR analysis in untreated, cisplatin, vitamin D and combination treated tumors. All experiments were performed in biological triplicate unless stated otherwise, +/- SEM, P *<0.5, P **<0.01, P ***<0.0001, P ****<0.00001.
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Treatment scheme for in vivo study. B. Mean tumor volume over the course of treatment in untreated, cisplatin, vitamin D and combination groups. C. Representative immunohistochemical (IHC) staining <t>of</t> <t>ALDH1A1,</t> RAR, RXR, <t>VDR</t> in tumors collected at the end of study, with corresponding quantification shown on the right (n=4), Scale bar, 100µm. D. Expression of stemness associated genes was determined by RT-qPCR analysis in untreated, cisplatin, vitamin D and combination treated tumors. All experiments were performed in biological triplicate unless stated otherwise, +/- SEM, P *<0.5, P **<0.01, P ***<0.0001, P ****<0.00001.
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vitamine d3 b o n  (Recordati)
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2D interaction of <t>Vitamin</t> <t>D</t> within the binding pocket of the PMF-related protein (PDB ID: 6NWD). The ligand forms predominantly hydrophobic and van der Waals interactions, with a characteristic π–σ interaction involving Tyr225, contributing to the strong binding affinity (–10.10 kcal/mol).
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Treatment scheme for in vivo study. B. Mean tumor volume over the course of treatment in untreated, cisplatin, vitamin D and combination groups. C. Representative immunohistochemical (IHC) staining of ALDH1A1, RAR, RXR, VDR in tumors collected at the end of study, with corresponding quantification shown on the right (n=4), Scale bar, 100µm. D. Expression of stemness associated genes was determined by RT-qPCR analysis in untreated, cisplatin, vitamin D and combination treated tumors. All experiments were performed in biological triplicate unless stated otherwise, +/- SEM, P *<0.5, P **<0.01, P ***<0.0001, P ****<0.00001.

Journal: bioRxiv

Article Title: Activation of vitamin D signaling suppresses platinum-induced ovarian cancer stem cell plasticity

doi: 10.64898/2026.09.22.753468

Figure Lengend Snippet: Treatment scheme for in vivo study. B. Mean tumor volume over the course of treatment in untreated, cisplatin, vitamin D and combination groups. C. Representative immunohistochemical (IHC) staining of ALDH1A1, RAR, RXR, VDR in tumors collected at the end of study, with corresponding quantification shown on the right (n=4), Scale bar, 100µm. D. Expression of stemness associated genes was determined by RT-qPCR analysis in untreated, cisplatin, vitamin D and combination treated tumors. All experiments were performed in biological triplicate unless stated otherwise, +/- SEM, P *<0.5, P **<0.01, P ***<0.0001, P ****<0.00001.

Article Snippet: Slides were incubated overnight at 4°C with antibodies against ALDH1A1 (CST, 54135), RARA (ProteinTech, 10331-1-AP), RXRA (Proteintech, 21218-1-AP) and VDR (CST, 12550).

Techniques: In Vivo, Immunohistochemical staining, Immunohistochemistry, Expressing, Quantitative RT-PCR, Analysis

2D interaction of Vitamin D within the binding pocket of the PMF-related protein (PDB ID: 6NWD). The ligand forms predominantly hydrophobic and van der Waals interactions, with a characteristic π–σ interaction involving Tyr225, contributing to the strong binding affinity (–10.10 kcal/mol).

Journal: Annals of Medicine

Article Title: Differential modulation of gentamicin antibacterial activity by vitamin B12 and vitamin D3 against Gram-positive and Gram-negative bacteria: an in vitro–in silico study

doi: 10.1080/07853890.2026.2653307

Figure Lengend Snippet: 2D interaction of Vitamin D within the binding pocket of the PMF-related protein (PDB ID: 6NWD). The ligand forms predominantly hydrophobic and van der Waals interactions, with a characteristic π–σ interaction involving Tyr225, contributing to the strong binding affinity (–10.10 kcal/mol).

Article Snippet: Vitamin D3 was used as Vitamine D3 B.O.N ® (oily solution, 200,000 IU/mL, corresponding to 5000 μg/mL; Bouchara-Recordati, France).

Techniques: Binding Assay